Glucose dehydrogenase pseudomonas sp characteristics

A glucose dehydrogenase of Pseudomonas sp accomplishment method, wherein: the addition of DNA absolute the coding arrangement declared in the Arrangement No. 1 accepting a glucose dehydrogenation action of the α subunit and the β-subunit as an electron alteration protein a bacillus acceptance to the brand Pseudomonas, to anatomy a transformant, culturing the transformant to aftermath a glucose dehydrogenase absolute said β subunit and afterwards the β-subunit of the additional glucose dehydrogenase.

A essentially antiseptic glucose dehydrogenase is disclosed. The agitator has action at a temperature of from about 30 ° C. to about 65 ° C. at a pH of from about pH 6 to about pH 10 and has an optimum action at a temperature from about 50 ° C. to about 60 ° C. at a pH of from about 8.5 to about 9.0.

The agitator is added characterized by application at atomic 90% balance action afterwards analysis at 50 ° C. for 15 minutes, getting NAD or NADP dependent, accepting a atomic weight of about 101,000 daltons as bent by gel filtration application TSK gel, accepting an isoelectric point of about 4.5 by ampholyte isoelectric focusing, and accepting a specificity for at atomic D-glucose and 2-deoxyglucose. The adopted antecedent of the agitator is Pseudomonas sp. FH1227.

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From: Biochemical Reagent